Oral / Poster Presentation File
1Department of Physical Education, Faculty of Humanities, University of Ayatollah Alozma Boroujerdi
2Department of exercise physiology, Faculty of Physical Education, University of Guilan
Aims. Therefore, this study aimed to investigate the impact of aerobic training on Parkin gene expression in skeletal muscle of old male rats.
Methods: Eight young male Wistar rats with eight to ten weeks of age and mean of 320 g in weight, and also 16 old rats with 16 to 18 months of age and mean of 450 g in weight were provided in the Biotechnology Center of Yazd Shahid Sadoughi University of Medical Sciences. Young rats were considered as Young-Control (n=8). The old rats were divided into Old-Control (n=8) and Old-Training (n=8). Old-Training rats were subjected to an aerobic training on a treadmill with 10° inclination for eight weeks (5 days per week). Rats run 30 min by 70% vVo2peak per session of training. The rats were sacrificed by injection of Ketamine-Xylazine mixture following 48 h after the final training session, and Soleus muscle was taken for examination. The gene expression of Parkin was determined by Real-Time PCR.
Results: The Old-Control displayed lower levels of gene expression of Parkin as compared to Young-Control (near 3-fold; P=0.02). Interestingly, the Parkin expression in the Old-Training group was not significantly lower than Young-Control (near 1.6-fold; P=0.09). Moreover, Parkin expressions were not significantly higher in Old-Training compared to Old-Control (near 1.4-fold; P=0.11).
Conclusion: The results indicated that Parkin expression, as a mitophagy marker, was reduced by aging. Interestingly, aerobic training could negate the reducing effect of aging in Parkin expression. These would suggest that aerobic training may prevent the old-inducing loss of mitophagy in skeletal muscle.
