| The Impact of Continuous Endurance Training and High-Intensity Interval Training on Fusion-Related Factors i.e. MFN1 and MFN2 in skeletal muscle of Wistar Rats |
| Paper ID : 1029-SSRC-13TH (R1) |
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Oral / Poster Presentation File |
| Authors: |
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Saeed Daneshyar *1, Fatemeh Jalali Moghim2 1Department of Physical Education, Faculty of Humanities, University of Ayatollah Alozma Boroujerdi 2Department of biology, Faculty of Basic Sciences, Bu-Ali Sina University |
| Abstract: |
| Background: Mitochondria is a dynamic organelle complicated by fusion and fission processes. Mitochondrial fusion results in an elongation of mitochondria and an expansion of its network (1). The key molecular regulators of the fusion are mitofusin 1 (Mfn1), mitofusin 2 (Mfn2) (2). It is established that endurance training can facilitate the mitochondrial dynamic (4). However, the training interventions of most previous studies were short-term and continuous bases and the effect of High-intensity interval training (HIIT) on the mitochondrial dynamics such as fusion has not been well explored. Aims: Thus, this study aimed to study the effect of CET and HIIT on fusion regulators i.e MFN1 and MFN2 in skeletal muscle of Wistar rats. Methods: Twenty-four male Wistar rats with a mean of nine weeks in age and a mean of 320 g in weight were provided in the Biotechnology Center of Shahid Sadoughi University. The rats were divided into three groups including Control (n=8), CET (n=8), and HIIT. The training-group rats were submitted by an exercise on a treadmill with 10° inclination for eight weeks (5 days/week). The CET-group rats run 30 min by 70% vVo2peak. The rats of HIIT were exercise-trained on six interval occasions so that they ran at 90% vVo2peak for 3-minutes followed by a 2-minute active recovery at 50% vVo2peak for 2-minutes. The gene expression of MFN1 and MFN2 in the Soleus muscle was determined by Real-Time PCR. Results: There were no significant differences in MT1 gene expression among studies groups (P>0.05). The gene expression of MT2 was higher in CET group as compared to Control (2.6-fold; P=0.03). However, the HIIT group did not show a significantly higher in MT2 expression as a comparison to control (1.4-fold; P=0.09). Moreover, it was not observed a significant difference between HIIT and CET groups in MFN2 (1.2-fold; P=0.13). Conclusion: The results indicated that MT2 expression, as a fusion marker, was increased by CET but not by HIIT. These would suggest that continuous endurance training compared to high-intensity interval training may be effective in the elongation of mitochondria and expanding the mitochondrial network. |
| Keywords: |
| Mitochondrial dynamic, Mitochondrial fusion, Mitofusin 2, Continuous endurance training, High-intensity interval training |
| Status : Abstract Accepted (Oral Presentation) |