| Inhibitory effect of one period of sub-maximal swimming Exercises in cell cycle stimulating genes(STAT3) in the pathways leading to the tumor in Lung tissue of Wistar Rats exposed to tobacco-derived carcinogens (NNK) |
| Paper ID : 1309-SSRC-13TH |
| Authors |
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mohammadbagher nikzad *1, Shadmehr Mirdar2, abolfazl aghababaeyan3 1University of Science and Technology of Mazandaran 2Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Babolsar, Iran 3General Department, School of Medicine, Golestan University of Medical Sciences and Health Services, Gorgan, Iran |
| Abstract |
| Background and Objectives: It is believed that sports programs have minimal impact on pathological changes in the lung tissue exposed to smoking carcinogens. The aim of this study was to investigate the effects of twelve weeks submaximal swimming exercise on the expression of STAT3 in lung tissue of rats following the exposure to the carcinogen NNK. Materials and Methods: Forty-six Wistar rats (with the average weight of 105.84 ± 27.93g) were divided into five groups: A) exercise(E), B) exercise-NNK(EN), C) NNK(N), D) control(C) and E)Vehicle group (V) group. Exercise groups completed swimming submaximal exercises for twelve weeks, five days per week (Water flow 4-10 L/min, 25-60min). Rats in Vehicle group and NNK treatment group were respectively administered subcutaneous injections of distilled water and NNK (12.5mg/kg, once a week, 12 weeks). 48 hours after the last exercise, rats died and samples of their lung tissue were collected and stored in -70ᵒ. STAT3 expressions were measured by Real time PCR-ABI method. One-way analysis of variance and Tukey post hoc test (P≤0.05) were used for data analysis. Results: There was a significant increase (p≤0.05) in the NNK group compared to E and E.N groups. There were Significant differences (P≤0.05) between E and EN groups with NNK group in the STAT3 gene expression while there was no significant difference (P≤0.05) between exercise groups with the control and saline groups. The submaximal swimming exercise prevented STAT3 gene expression in the lung tissue of rats following the exposure to the carcinogen NNK. Conclusion: It seems that by reducing the STAT3 gene expression in the exercise groups, submaximal training can be used as a complementary therapy method along with other methods to prevent main factors leading to tumors. |
| Keywords |
| Submaximal exercises, NNK, STAT3 |
| Status: Abstract Accepted (Oral Presentation) |
