Oral / Poster Presentation File
1University of Science and Technology of Mazandaran
2General Department, School of Medicine, Golestan University of Medical Sciences and Health Services, Gorgan, Iran
3Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Babolsar, Iran
Materials and Methods: 86 Wistar rats were divided into 8 groups of 10 including training group, NNK group, NNK- training group, supplement group, NNK-supplement group, training-supplement group, training- NNK-supplement- group, vehicle group injected with distilled water and a group of 6 as control group. Swimming exercises were performed for 12 weeks, 5 days a week. The injection of NNK and distilled water was performed subcutaneously once a week at 12.5 mg / kg for 12 weeks. Afterwards, the lung tissue samples were taken and the expression level of gene signal transducer and activator of transcription-3 was measured using Real time PCR-ABI method. Data comparison was performed through one-way analysis of variance and Tukey test at p≤0.05.
Results: Comparison of the expression of STAT3 gene in NNK group with three groups of NNK-supplement group, NNK- training group, training- NNK-supplement- group revealed a significant reduction (p≤0.05) in the expression of this gene compared to NNK. The expression of STAT3 gene in lung tissue exposed to NNK carcinogen was significantly reduced following swimming exercises and black seed nanocapsules administration.
Conclusion: Seemingly, the endurance training and black seed nanocapsules administration, along with other treatments, could reduce the carcinogenic impact of NNK and mitigate the negative effects of smoking by reducing the STAT3 inflammatory agent.
