| The Effects of Aerobic Training and Omega-3 Intake on Aβ42, Neprilysin and γ- Secretase in the Hippocampus of Male Rats Alzheimer's model |
| Paper ID : 1528-SSRC-13TH |
| Authors |
|
Ali Yaghoubi *1, reza arefi gharari2 1Department of Physical Education and Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran 2Assistant Professor Department Sport physiology, Binalod Institute of Higher Education, Mashhad, Iran |
| Abstract |
| Alzheimer disease (AD) is characterized by excessive deposition of the amyloid-β peptide (Aβ) in the central nervous system and reducing its level is the goal of many medications. Thus the aim of this study was to investigate the effect of aerobic training and omega-3 intake on Aβ42, Neprilysin and γ- secretase in the hippocampus of male Rats Alzheimer's model. 50 male Wistar rats (12weeks-old and weight 222.31±11.91 g), divided into 5 groups including, control Alzheimer's (AC), Alzheimer's with omega-3 intake (AO), Alzheimer training (AT), Alzheimer's with omega-3 intake and Alzheimer training (AOT) and Healthy Control (HC). Alzheimer's disease induced by injection of homocysteine (60mM) into the rat brain ventricle. Training with a speed of 20 m/min (60 minutes and 5 day/week on the treadmill) was applied. The supplement group, was received Omega-3 supplement 800mg/kg of body weight, daily for eight weeks. Level of Aβ42, Neprilysin and γ- secretase protein measured using ELISA method. In data analysis, a one-way analysis of variance and Tukey test as a post hoc, were used (P<0.05). The obtained results showed that the level of Aβ42 in hippocampus of AC was significantly higher than HC group (P=0.001). Also the level of the Aβ42 in hippocampus of AC was significantly higher than AO, AT and AOT groups (P values 0.001, 0.007 and 0.003 respectively). Also the γ- Secretase level in hippocampus of AC was significantly higher than HC group (P=0.001). Also the γ- secretase levels in hippocampus of AC group was significantly higher than AO, AT and AOT groups (P values, 0.002, 0.001 and 0.001 respectively). There was no significant difference between Neprilysin level of the hippocampus of research groups (P = 0.534). it appears that exercise training and omega-3 consemption, can prevent amyloidgenic pathways by reducing the level of γ- secretase, and lead to reducing the level of Aβ hippocampus of Alzheimer's disease subjects. In total, aerobic exercise training and omega-3 intake can be studied as a complementary therapy in Alzheimer's patients. |
| Keywords |
| Aerobic Training, Omega-3 Intake, amyloid-β42, Neprilysin, γ- Secretase, Alzheimer's disease |
| Status: Abstract Accepted (Poster Presentation) |
