| The comparison of effect of high intensity interval and continues training on skeletal muscle sestrin2 in old rats |
| Paper ID : 1529-SSRC-13TH |
| Authors |
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Ali Yaghoubi *1, Saghar Delshad2, Najmeh Rezaeian3 1Department of Physical Education and Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran 2Department of Physical Education and Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran. 3Department of physical education, Bojnourd branch, Islamic Azad University, Bojnourd, Iran |
| Abstract |
| Background and Objective. Sestrin2 is known as the markers involved in progression of aging. Exercise training, on the other hand, is known to be one of the most important modulators of aging, however, the effects of high intensity interval training exercise (HIIT) on Sestrin2 are less well known. Therefore, the aim of this study was to compare the effect of high intensity interval and continuous training on skeletal muscle sestrin2 in elderly rats. Materials and Methods. Thirty old male Wistar rats (18 months) were randomly divided into control (C) (n=10), high intensity interval training (HIIT) (n=10), and continuous training (MICT). After estimating the maximum speed, rats in HIIT group participated in 8 weeks of high-intensity interval training of running on the treadmill (5-8 sets of running for 2 minutes at intensity of 80-100 VO2Max with rest intervals of running for 2 minutes at intensity of 50 VO2Max) five sessions per week and rats in MICT groups participated in continuous training protocol included (8 weeks/ 3 sessions per week with 65 to 70% VO2max for 40 min) running on a treadmill. Skeletal muscle tissue samples were extracted 48 hours after the last training session to measure protein levels of factors assessed. Independent t-test was used to analyze the data and P<0.05 was considered significant. Results. The results showed that HIIT resulted in a significant increase in sestrin2 levels compared to the control group (P<0.05). However, MICT protocol had no significant effect on the protein values of sestrin2 (P <0.05). Also, there was no significant difference between the two training groups (P <0.05). Conclusion. It seems that HIIT can play more effective role in increasing the protein levels of sestrin2, which plays a central role in progression of aging. |
| Keywords |
| High Intensity Interval training, mild intensity continuous training, Autophagy, Sestrin 2, elderly rat |
| Status: Abstract Accepted (Poster Presentation) |
