| The Effect of 8-Week Aerobic Training on TRPC6, PMCA4b levels and Pathological Cardiac Hypertrophy in Middle-Aged Wistar Rats |
| Paper ID : 1796-SSRC-13TH |
| Authors |
|
Behrouz Baghaiee * Department of Physical Education and Sport Science, Jolfa Branch, Islamic Azad University, Jolfa, Iran |
| Abstract |
| Objectives and aim: Cardiac aging is often accompanied by adverse structural and functional changes such as increased left ventricular wall thickness (hypertrophy) and compromised ventricular contractility, termed pathological cardiac hypertrophy. One of the factors affecting pathological cardiac hypertrophy is the amount of intracellular calcium. The Transient receptor potential canonical 6 (TRPC6) is a Ca2+-permeable cation channel presenting in the plasma membrane of many tissues, including the heart tissue. Unlike TRPC6, plasma membrane Ca2+ ATPase (PMCA) is a transporter protein in the cellular plasma membrane that maintains an appropriate cytoplasmic Ca2+ level via removing Ca2+ from the cell. This study aimed to investigate the effect of moderate aerobic exercise on TRPC6 and PMCA4b and dimensions of the heart in the middle-age Wistar rats. Materials and Methods: Forty Wistar rats including young (n=10, 4-month-old) and middle-aged rats (n=30, 13-15 months old) were included in this experimental study. All young and 10 middle-aged rats did not exercise and served as a control comparision; while the remaining 20 middle-aged rats were trained at moderate intensity for 4-week (n=10) or 8-week (n=10) on a treadmill. Results: PMCA4b and Left ventricular internal diameter (LVID) were significantly decreased (P=0.001) while TRPC6 and Left ventricle wall thickness (LVWT) levels were remarkably increased (P=0.001) in the heart tissue of middle-aged compared to the young rats. Aerobic training significantly decreased TRPC6 (P=0.001) in the middle-aged-T4 and T8 groups (P=0.001) compared to the control non- middle-aged rats. However, aerobic training had no effect on PMCA4b protein expression of in the middle-aged-T4 and T8 groups compared middle-aged control groups. LVID was significantly increased in eight weeks, and LVWT was significantly reduced in the eighth week (P=0.011 and P=0.028 respectively). Conclusions: Aerobic training attenuates middle-aged induced pathological cardiac hypertrophy by attenuating TRPC6. |
| Keywords |
| Key words: TRCP6, PMCA4b, Pathological cardiac hypertrophy |
| Status: Abstract Accepted (Oral Presentation) |
