Effect of different intensities on muscle expression of Dcn muscle in rats |
Paper ID : 1837-SSRC-13TH |
Authors |
Elham Vosadi *, Farhad Gholami, Elham Mortazavi Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahroud University of Technology, Semnan, Iran |
Abstract |
Skeletal muscle as a secretory organ and myokines as agents derived from skeletal muscle can be affected by proper exercise. The aim of the present study was to investigate a period of exercise activity with different intensities on Dcn gene expression in rats. In this experimental study, Twenty-four male adult Wistar rats, were divided into three groups: (1) High intensity Training, (2) Low intensity Training and (3) Control groups. Training groups trained for 8 weeks and 5 days a week. The HIT group exercise program consists of running on a treadmill with five alternatives (8 minutes with 85-90% VO2max intensity and 2 minutes 50-60% VO2max intensity) and the LIT group exercise program is included five alternatives (8 minutes with 55-60% VO2max intensity and 2 minutes 45-50% VO2max intensity), that did a specific hour of the day and at the same time, the control group had no exercise. At first, the Soleus muscle homogenates and the expression of Dcn genes were measured by Real- time PCR analysis. The data were analyzed by one-way ANOVA and Tukey post hoc test and statistical differences were considered significant at P<0.05.The results of this study, showed that the expression levels of Dcn gene in the high intensity interval training group were significantly higher than the control group (P = 0.001), while the levels of Dcn in the low intensity interval training groups were not significantly different than the control group (p = 0.11). According to the results of the present study, it seems that High-intensity exercise can lead to a greater increase in muscle growth than low-intensity exercise. |
Keywords |
Dcn,High intensity Training, Low intensity Training, male adult rat. |
Status: Abstract Accepted (Poster Presentation) |